Gloves: they make a difference. But one between "happy" and "miserable", probably not. But do get them if you intend to be riding any more after that 35-miler (which I do not doubt you will!!).

Chamois butt'r: I use it only when I ride many days in a row, especially rides on very hot days when I'm so sweaty my chamois just can't take all the humidity out. That's the only time when it gets necessary. That being said, I don't use chamois butt'r but "zincofax", which is the Canadian name of the stuff you put on the butt cheeks of babies to avoid diaper rash. Works wonders on big babies, too, available everywhere, not so expensive.