+1 on checking into your local bike club(s)...that way, you'll have some opportunities to ride with others, as your event ride is surely with a larger group. It's always good to have some experience in riding on roads, in traffic with others, learning and practicing correct road signals, stopping/starting up, crossing R/R tracks, even minor roadside maintenance, as in changing a flat tire ahead of time, so that it's incorporated into your preparation.
Most clubs have rides scheduled for new riders and are led by a trained (volunteer) ride leader, experienced in and willing to continue leading novices so that they won't ride off and drop you or go too fast or on difficult terrain either. Look for social-paced rides on easy/flat routes with low mileage to begin, then show up, learn from others and have fun!
Also, I had a couple of decades on you when I decided to participate in my first AIDS fundraising ride. It was for a 4-day, 50 miles/day (200 total with MUCHO hills!) ride and I had a big ol' heavy hybrid, sneakers/toeclips, tshirts...but I did invest in a helmet, cycling shorts, cycling gloves and water bottles as was wisely suggested here, plus I went to my LBS' free flat tire repair session, so I was all set. Yes, it was hard, but I trained well for it and it was an amazing experience (fyi: that was about 40k miles and $100k plus ago!).
Go for it!
Tailwinds!
Mary




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because unlike your one day ride .... you gotta do it again. 