Don't know if this is your problem, but ITB Syndrome is rampant among cyclists. Men and Women alike. Without seeing your fit, it is difficult to tell you what to do on your fit, but often times, dropping your seat a tiny bit will help. Unless your seat height is WAY OFF, never change your seat height by more than 1/4 inch at a time! You want to slowly change the height or you may find that you have other problems crop up.

I find that yoga helps a lot - basically, you get to stretch that IT band and loosen it up and that is all it takes. Do yoga regularly, try to get your seat height figured out and you should be doing great! The pigeon position in yoga really helps the ITB tightness.

Remember, that in winter and summer sometimes you have to change your seat height a little. I always have more trouble in the winter with ITB Syndrome. One reason is the muscles are tighter, one reason is that I'm wearing tights which makes me sit higher on the saddle, and one reason is that I'm not riding as much as it is that first of the season settling in and getting fitter. So, I drop my saddle in the winter a 1/4 of an inch or so.

Hope it gets better for you.