ok - this is just gonna blow everyones mind but I wash mine based on miles. Since I"m a bike commuter and I avg 12 miles a day, I wear my shorts for 2 days - or 24 miles. If I do a noon ride then I wash my shorts every day since a noon ride is 10-15 miles or 22-27 miles that day. I do wash them in cold water and hang them to dry and I use Tide since that's the only thing that seems to keep the shorts and jerseys smelling fresh. I own 6 pairs of long shorts and 4 pairs of short shorts so I have shorts for the weekend just in case I do use a pair every day (or I've gone over a week without doing laundry {see the how many jerseys is too many thread lol}) To date I haven't had any issues with yeast infections or saddle sores caused by wearing a pair of shorts for more than 1 day. I've even - - taken Fridays commuter shorts which have only been worn for 12 miles and worn them for a 20+ ride on Saturday before throwing them in the wash.