How tall are you? Will you be riding mountain or road?
If you are tall enough, men's bikes might be better for you because men's bikes tend to hold more weight. But, best to find a local bike shop with a certified bike fitter. Then, you don't have to worry as much about getting the wrong bike.
There are also lots and lots of websites for plus, plus, plus size biking gear (jerseys/biking shorts) if you don't fit anything in the store.
Lots of TE members have lost weight and gained athleticism via biking. As for riding a bike in more than 25 yrs, me too. I just started myself at the age of 35++ years old. You'll pick it up. But you'll have to try riding in order to remember how to do it. So don't give up!
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2012 Specialized Amira Elite, upgraded carbon handle bars, Jett saddle 143mm switched to 145mm 2012 Selle Italia Max SLR Gel Flow saddle
2011 Specialized Ariel Sport,suspension post,Serfas Rx Women's Microfiber saddle



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