My biking shorts do not have a chamois or any other padding. I found that, given my riding position, the extra padding increased the pressure on my "girly bits". To avoid chaffing, I wear some very tight yet breathable underwear so that my shorts cannot rub on the "girly bits".

What works for one may not work for all - it depends on your riding position, the hardness of your saddle, and the sensitivity of your body.