The past few rides, I have developed pain in my right knee that presents itself the next day. When I wake up in the morning, the knee is sore when it supports weight. Wearing my running shoes with my insoles (orthodics) helps this pain go away.

I did get a new bike and have only noticed this problem after I started riding it but I transferred my pedals from my old road bike and haven't changed the cleat alignment on my shoes. The seatpost has also not been clamped in tight enough my last 2 rides and so I readjusted it and tightened it significantly.

Any thoughts regarding this or ideas of what may be causing it?