bouncey, lots of things could make a difference....
humidity, how hydrated you are, what point in your cycle you're in, if you've taken ibuprofen (yes, that could cause the blood to reach the surface a bit easier...), also, could you saddle have changed position somehow? even a slight change could make a difference. how about your shorts? same shorts? same detergent? were they rinsed well enough?
I've never figured out, other than leaving the shorts on after a ride - yup, they come off almost immediately! - what exactly causes that....roughness...
but my cure for it, when it happens is Jason Tea Tree Gel - it's an aloe vera gel with Tea Tree, chamomile and arnica. very soothing and healing!
good luck!
Namaste,
~T~



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