Naw. I never worry about my bike. I have even dropped it off the day before the race and I didn't worry about it.
Of course, the key to not worrying... is not having the nicest bike in transition.
The only thing I have worried about losing out of transition is my wetsuit. People have been known to carry off the wrong one.
"Life is not a journey to the grave with the intention of arriving safely in a pretty and well preserved body, but rather, to skid in broadside thoroughly used-up, totally worn out, and loudly proclaiming: WOW WHAT A RIDE!!!!"