My $0.02 worth (which are worth less than those of more experienced riders) ...

I went saddle-shopping with my daughter last week. You'd be amazed at the difference between saddles! My "comfort" saddle doesn't feel so comfortable either any more, now that I've found a narrow one that fits my sit bones and isn't in the way of my inner thighs when pedalling. So I suggest you find a good bike shop where you can try some different saddles. It may be that the comfort seat is simply too wide. (I'm just looking for a bike now that deserves that saddle I found )

Also ... chamois is very different from garment to garment, so try some different shorts while you're at it and find one with a chamois that stays put, doesn't bunch up, and doesn't interfere with your spin motion. There ARE different +-sized shorts out there. I know, 'cuz I wear 'em too.

And finally, yes, chamois butter does help. I've got that on my shopping list now. I've been ok for short rides without, but I have a longer ride coming up and, thanks again to more experienced riders (that'd be you, Trek420), I know I'll need chamois butter for that one.