Chamois Butt'r, etc

[XMcShiftersonX]

So as everyone else has said - Yes, NO underwear with padded cycling shorts.

I would suggest a thickly padded pair of shorts, like Assos. I just purchased my second pair for STP so I can have a clean pair each day. I didn't start to notice chaffing in the nether regions until I went on rides longer than 50+ miles. What I do now is bring a tube of DZ Nuts (my current favorite chamois cream) on all of my long rides, I slather it on the chamois before I begin riding from the part that touches my butt all the way up to the front of my labia and out to the sides of my chamois. I tend to have most pain up towards my labia if I have any. I found that reapplying every time I go to the bathroom on my ride has helped. You should try this out before STP, so on all your long rides from now on, I would try it, so you know what works and doesn't. You don't want to figure it out on a 200 mile ride.