If you wear knickers under your chamois shorts, you'll find they bunch up and chafe and rub
Wear nothing and just make sure you get out of them asap after a ride and wash and dry them well
Avoid wearing them two rides in a row without a wash whenever possible
Lots of discussion here about creams, cremes, butters etc to make things more comfortable, and also about seat height and position too
Your tour sounds super, keep us posted!
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R



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