Was the fore/aft position of the saddle changed? It sounds like you aren't sitting on the saddle as intended by the design.
Even if it wasn't moved on the post (seat post bolt to me means the bolt that keeps the post from sliding into the frame) all the changes you mention affect your reach. You could unconsciously be accomodating by moving foreward or backward on the saddle, or tipping your pelvis, or rocking your hips, or so many things.
And, are you wearing underwear? Maybe the change finally put you in the right spot for you, but not for any underwear.



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