I ride the C&O on cyclocross bike with either slightly knobby 700x30's or semi-slick 700x32's and it's fine. But then, I've been riding there for years and am used to the surface, and I'm not usually loaded down with panniers and stuff.
A lot of it depends on how many miles you're going to ride each day, how much gear weight you're hauling, and how much bumpiness and vibration you can tolerate. Can you do a few day trips on each bike fully loaded to see which is more comfortable for you? Also check out the Mid-Atlantic portion of the forum, a bunch of us get together for somewhat frequent C&O rides, including the ladies who did the thru-ride last year. Joining us for a ride or two (or three) would be a great way to test your bikes and get some advice.



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) would be a great way to test your bikes and get some advice.

